Polyene-Resistant Mutants of Aspergillus fennelliae : Sterol Content and Genetics
نویسندگان
چکیده
منابع مشابه
Polyene-resistant mutants of Aspergillus fennelliae: identification of sterols.
Four mutants strains of Aspergillus fennelliae resistant to various polyene antibiotics have been analyzed for their sterol content. The mutant strains contained Delta(7,22,24(28))-ergostatrien-3beta-o1 and Delta(7,22)-ergostadien-3beta-o1 as the major sterols, whereas the wild types contained ergosterol, indicating that the mutants contain metabolic blocks for C(5)-C(6) dehydrogenation and C(2...
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Mutants of the genes nys1 and nys3 differ from sensitive strains (nys(+)) in their sterol content. Ultraviolet absorption spectra of the nonsaponifiable material extracted from cells of nys(+) demonstrated the presence of ergosterol and 24(28)-dehydroergosterol. In nys1 mutants, the spectrum suggests the presence of a new sterol. The absorption spectrum of extracts from nys3 mutants indicates a...
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Mutants of Saccharomyces cerevisiae have been selected for resistance to the polyene antibiotics, etruscomycin, filipin, nystatin, pimaricin and rimocidin. All mutants are resistant to nystatin and there are several patterns of cross-resistance. The mutants were allocated to four unlinked genes pol I , 2, 3 and 5. A fifth gene, pol 4, was recovered as a double mutant with a strain carrying the ...
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Six mutants of Cryptococcus neoformans resistant to nystatin and pimaricin and three mutants resistant to amphotericin B were isolated by ultraviolet irradiation techniques from two wild-type strains. The major sterols of the wild-type strains were Delta(7)-ergosten-3beta-ol and ergosterol. All six mutants resistant to nystatin and pimaricin showed either loss of ergosterol and concurrent produ...
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Recently, we showed that the effect of dipole modifiers (flavonoids and styrylpyridinium dyes) on the conductance of single amphotericin B (AmB) channels in sterol-containing lipid bilayers primarily resulted from changes in the membrane dipole potential. The present study examines the effect of dipole modifiers on the AmB multi-channel activity. The addition of phloretin to cholesterol-contain...
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ژورنال
عنوان ژورنال: Antimicrobial Agents and Chemotherapy
سال: 1974
ISSN: 0066-4804,1098-6596
DOI: 10.1128/aac.6.1.102